cell culture human sarcoma cell lines ht1080 (ATCC)
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Cell Culture Human Sarcoma Cell Lines Ht1080, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc"
Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc
Journal: American Journal of Translational Research
doi:
Figure Legend Snippet: 72-hour treatment with doxorubicin. Top graphs represent (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell proliferation compared to an untreated control. Significance is denoted by (*) such that P-values are represented by P<0.05 (*), P<0.005 (**), P<0.0005 (***), and P<0.0001 (****). Bottom graphs represent dose-response curve of (E) HT1080, (F) SW872, (G) VAESBJ, and (H) SW982 cell lines with associated IC50, calculated using Prism GraphPad 7.
Techniques Used: Control
Figure Legend Snippet: Efficacy of 72-hour treatment with doxorubicin alone and in combination with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cells measured through proliferation compared to untreated controls. Data are presented as mean ± SD.
Techniques Used:
Figure Legend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell lines. The dotted lines represent thresholds for Chou-Talalay antagonism (CI>1.1) as well as synergy (CI<0.9). Additive effect corresponds to (0.9
Techniques Used:
Figure Legend Snippet: Combination Index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin
Techniques Used:
Figure Legend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin
Techniques Used:
Figure Legend Snippet: Mucin depletion as assessed quantitatively through ImageJ RGB analysis
Techniques Used: Control
Figure Legend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC1 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC1 staining, respectively. Scale bar: 50 μm. Final magnification =600×.
Techniques Used: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining
Figure Legend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC4 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC4 staining, respectively. Scale bar: 50 μm. Final magnification =600×.
Techniques Used: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining
Figure Legend Snippet: Western blot analysis of a panel of biomarkers for apoptosis, cell cycle progression, and autophagy in soft tissue sarcoma HT1080 and SW872 cells.
Techniques Used: Western Blot
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